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Five spectra per peak were recorded at the up-slope, apex and down-slope of each peak within the UV wavelength range of nm, step size 0. The HPLC chromatogram of each extract was loaded in the offl ine mode and UV spectra of the peaks under consideration were loaded.

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The library was representative of phenolics identifi ed in the literature for the plants under study. Initial matches were performed in the plant extracts for the identifi cation of markers, on the basis of retention time and UV spectrum. The peak purity was calculated with average spectra and a similarity curve was used to confi rm the purity wherever needed.


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After initial profi ling by performing library matches, the fi nal identifi cation of selected markers was corroborated by comparing the frag-mentation patterns with those of authenticated standards in all cases. The calibration was automatically updated for each sequence analysis and each analysis was performed using identical analytical conditions. Recovery experiments were performed with chlorogenic acid as an initial assessment of the extraction effi ciency. This was achieved by adding aliquots of standard chlorogenic acid 0. Spiked and unspiked samples were extracted and analysed according to the method described in the sample preparation section.

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Additional experiments were carried out with three markers chlorogenic acid, luteolinO-glucoside and apigeninO-glucoside in FE in triplicate to determine limits of detection LOD; signal to noise ratio 3 : 1 , limit of q Dried herbs can be used in a Documents. Recommendations on Herbs and Herbal Formula in? It is common… Documents. Herbs and herbal remedies Education. Dealers of Herbal Cosmetics, Products and extracts New Delhi, India. Email: npcs.


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